Create a checkbox

    chk_my_checkbox = IntVar()
    chk_my_checkbox = tk.Checkbutton(root, text="Enable Calibration",variable=chk_my_checkbox_value, onvalue=1, offvalue=0, command=chk_my_checkbox_clicked)
    chk_my_checkbox.place(x=20, y=20)

Get checkbox value

    if (chk_my_checkbox_value.get() == 0):